10x barcoded gel beads  (10X Genomics)

Journal: iScience

Article Title: C5aR1-positive adipocytes mediate non-shivering thermogenesis in neonatal mice

doi: 10.1016/j.isci.2024.111261

Figure Lengend Snippet:

Article Snippet: The sorted single-cell suspension, 10x 3′ barcoded gel beads, and oil were loaded into Chromium Single Cell G Chip to capture single cells in nanoliter-scale oil droplets by Chromium Controller and to generate Gel Bead-In-EMulsions (GEMs) and single-cell RNA-seq libraries were obtained following the 10x Genomics protocol using reagents included in the Chromium Single Cell 3′ v3.1 Reagent Kit.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, SYBR Green Assay, Sequencing, Software

Journal: bioRxiv

Article Title: Regulation of transposons within medium spiny neurons enables molecular and behavioral responses to cocaine

doi: 10.1101/2024.08.28.610134

Figure Lengend Snippet: a) Timeline of single-nuclei RNA sequencing (snRNAseq) experiment. Male and female mice (n=15/sex/treatment) were delivered synthetic ZFP189 transcription factors (TFs) via stereotaxic surgery to the NAc. Mice then received seven sequential days of cocaine (10mg/kg I.P.) injection and underwent locomotor activity testing before tissue harvesting, NAc dissection, nuclei purification, and snRNAseq on the 10X Genomics platform. b ) Heat map of cell-specific marker genes across all identified clusters. c) Global clustering across experimental treatment groups. NAc nuclei identifies all major classes of the mouse NAc, including Drd1+ and Drd2+ MSNs. UMAP, Uniform Manifold Approximation and Projection. d) ZFP189 TF variants similarly identify all major cell types of the NAc. e) Dot plot representing the average and percent expression of nuclei expressing marker genes of each identified cell-type of the NAc. f) Histogram of DEGs within all NAc cell types by ZFP189 TF treatment. DEGs were generated relative to the ZFP189 NFD control, with an absolute log 2 (fold change) > 0.25 with a P -adjusted value of < 0.05.

Article Snippet: The nuclei suspension was loaded into a Chromium Chip B with partitioning oil, reverse transcription reagents, and gel beads containing 10X Genomics barcodes.

Techniques: RNA Sequencing Assay, Injection, Activity Assay, Dissection, Purification, Marker, Expressing, Generated, Control